Flow cytometric analysis has become a common tool in experimental biology as well as in clinical sciences. There are several flow cytometry software, both freeware and commercial. However, these are not open ended. They can only perform a set of statistical analysis, and can only generate a particular set of graphical output. Unfortunately, the data generated by a flow cytometer can not be read by standard data analysis software. This limitation can be overcome, if one can extract the whole or a selective data set from a flow cytometry experiment in a suitable spread sheet format (i.e. tab delimited format). FlowPy is such a tool. And more.
What can you do using FlowPy:
1. Visualize your data in 1 or 2-D (histogram or dot plot) and then select a particular region of your data set.
2. For higher dimensional data, you can do clustering and then select a specific cluster.
3. Extract the selected data and export to a text file or to any standard spreadsheet software.
FlowPy can read and extract data from Flow Cytometry data files saved in Listmode format following Flow Cytometry Standard (FCS) 2.0/3.0.
FlowPy has been written in Python. The GUI version works only in Windows.
Citation information: Please cite FlowPy in publication as 'FlowPy (http://flowpy.wikidot.com)'
Credit: Soumitra Saxena, M. V. Seetha Rama Sastry, Revanth Sai Kumar, Tejas Mehta, Biplab Bose, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, India